Department of Veterinary Pathobiology

Elizabeth BrydaElizabeth Bryda
Professor
Director, Rat Resource and Research Center (RRRC) (www.rrrc.us)
Director, MU Animal Modeling Core (AMC)
(https://research.missouri.edu/amc)
Co-Director, Comparative Medicine Program (https://cmp.missouri.edu/)

  • M.S. Microbiology – Rutgers University/University of Medicine & Dentistry of NJ
  • Ph.D, Rutgers University/University of Medicine & Dentistry of NJ
  • Postdoctoral Training: Molecular Genetics - Wadsworth Center, Albany, NY

Building Address: Room N131, 4011 Discovery Drive, Columbia, MO 65201 (Discovery Ridge Research Park)
Phone Number: 573-882-5504
Email: brydae@missouri.edu

Research Emphasis: In my laboratory, we are interested in genetic engineering, molecular genetics and animal modeling. We take a comparative approach to studying human disorders by using a variety of animal models of disease, including both rodent and zebrafish models. We are interested in characterizing disease-causing genes/disease variants and their protein products in order to elucidate the molecular pathways in which these genes/proteins participate. This knowledge will allow a better understanding of both normal and abnormal development/function and may ultimately lead to targeted therapeutics. We have generated and characterized a variety of animal models to study diseases including polycystic kidney disease, hereditary deafness, Alzheimer’s Disease, epilepsy, cancer, cardiovascular disease and inflammatory bowel disease. Additionally, we are interested in developing and using state-of-the-art genetic engineering methods to generate and characterize new animal models. For example, our lab has unique expertise working with rat embryonic stem cells (ESCs), and we have isolated new rat ESC lines.  We have helped validate a novel rat and zebrafish cell ablation model system and we use evolving technologies such as CRISPR/Cas9 to create new genetically engineered animal models.  Genome editing has allowed us to create rodent models that carry rare human genetic alterations in order to validate these putative disease-causing mutations and generate animal models that can facilitate personalized medicine interventions. We are also interested in studying the role of non-genetic factors on animal model phenotypes. Examples of this include exploring the impact of the microbiome on zebrafish to study stress and anxiety, investigating microbiota sex differences in an Alzheimer’s Disease rat model, and evaluating the impact of probiotics and plant-based dietary supplements in a variety of animal model systems.

Teaching Responsibilities: BSC 4982WI/7982: Human Inherited Diseases (Course Director and sole instructor); LAB AN 9476: Grant and Manuscript Writing for Biomedical Researchers (Course Director, primary instructor); VPB 4787: Historical, Societal & Ethical Topics in Medicine & Biomedical Research (Course Director, sole instructor); LAB AN 9498: Lab Animal Biology (instructor for genetics section).

Selected Publications:

Men, H., Hankins, M.A., Bock, A.S., Beaton, B.P., Davis, D.J., Chesney, K.L., Bryda, E.C. Mutational analyses of novel rat models with targeted modifications in inflammatory bowel disease susceptibility genes. Mamm Genome. 2021 Jun;32(3):173-182 (2021).

Chesney, K.L., Men, H., Hankins, M.A., Bryda, E.C. The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats. Physiol Genomics. 53(6):269-281 (2021).

Men, H., Stone, B.J., Bryda, E.C. Media optimization to promote rat embryonic development to the blastocyst stage in vitro.Theriogenology 151:81-85 (2020).

Stone, B.J., Steele, K.H., Men, H., Srodulski, S.J., Bryda, E.C., Fath-Goodin, A. A Non-surgical Embryo Transfer Protocol Using the Rat NSET™ Device for Fresh and Cultured Blastocysts in Rats.J Am Assoc Lab Anim Sci 59:488-495 (2020).

Chesney, K.L., Chang, C., Bryda, E.C. Using vaginal impedance measurement to stage estrous in rats given luteinizing hormone releasing hormone (LHRH) agonist. J Am Assoc Lab Anim Sci 59:282-287 (2020).

Bryda, E.C ., Men, H., Davis, D.J., Bock, A.S., Shaw, M.L., Chesney, K.L., Hankins, M.A. A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression. Sci Report 9:13330 (2019).

Shimoyama, M., Smith, J.R., Bryda, E ., Kuramoto, T., Saba, L., Dwinell, M. Rat Genome and Model Resources. ILAR J. 58:42-58 (2017).

Walters, E.M., Wells, K.D., Bryda, E.C. , Schommer, S., Prather, R.S. Swine models, genomic tools and services to enhance our understanding of human health and diseases. Lab Anim (NY). 46:167-172 (2017).

Li, S., Lan, H., Men, H., Wu, Y., Li, N., Capecchi, M.R., Bryda, E.C. , Wu, S. Derivation of Transgene-Free Rat Induced Pluripotent Stem Cells Approximating the Quality of Embryonic Stem Cells. Stem Cells Transl Med. 6:340-351 (2017).

Davis, D.J., Doerr, H.M., Grzelak, A.K., Busi, S.B., Jasarevic, E., Ericsson, A.C., Bryda, E.C.Lactobacillus plantarum attenuates stress-induced responses in adult zebrafish via the gut-brain axis. Scientific Reports 6:33726 (2016).

Hanson, M.M., Liu, F., Dai, S., Kearns, A., Qin, X., Bryda, E.C. Rapid conditional ablation model for hemolytic anemia in the rat. Physiological Genomics 48:626-632 (2016).

Ericsson, A.C., Akter, S., Hanson, M.M., Busi, S.B., Parker, T.W., Schehr, R.J., Hankins, M.A., Ahner, C.E., Davis, J.W., Franklin, C.L., Amos-Landgraf, J.M., Bryda, E.C. Differential Susceptibility to Colorectal Cancer due to Naturally Occurring Gut Microbiota. Oncotarget 6:33689-33704 (2016).

Taskiran, E.Z., Korkmaz, E., Gucer, S., Kosukcu, C., Kaymaz, F., Koyunlar, C., Bryda, E.C. , Chaki, M., Lu, D., Vadnagara, K., Candan, C., Topaloglu, R., Schaefer, F., Attanasio, M., Bergmann, C., Ozaltin, F. Mutations in ANKS6 Cause a Nephronophthisis-Like Phenotype with ESRD. J Am Soc Nephrol. 25:1653-1661 (2014).

Bock, A.S., Leigh, N.D., Bryda, E.C . Effect of Gsk3 inhibitor CHIR99021 on aneuploidy levels in rat embryonic stem cells. In Vitro Cell Dev Biol Anim. 50:572–579 (2014).

Men, H. and Bryda, E.C. Derivation of a Germline Competent Transgenic Fischer 344 Embryonic Stem Cell Line.  PLoS One  8:e56518 (2013).

Bryda, E.C. , Johnson, N.T., Ohlemiller, K.K., Besch-Williford, C.L., Moore, E., Bram, R.J. Conditional Deletion of Calcium-modulating Cyclophilin Ligand Causes Deafness in Mice.  Mamm Genome 23:270-276 (2011).

Bouvrette, D.J., Sittaramane, V., Heidel, J.R., Chandrasekhar, A., Bryda, E.C. Knockdown of Bicaudal C in Zebrafish (Danio rerio) Causes Cystic Kidneys: A Non-Mammalian Model of Polycystic Kidney Disease. Comparative Medicine 60:96-106 (2010).

Bryda, E.C . and Bauer, B.A. Chapter 20: A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites. In “Methods in Molecular Biology, Rat Genomics: Gene identification, Functional Genomics and Model Applications.” I. Anegon (ed). Humana Press, Totowa, NJ. Methods Mol Biol. 597:287-299 (2010).

Stagner, E.E., Bouvrette, D.J., Cheng, J., Bryda, E.C. The Polycystic Kidney Disease-related Proteins Bicc1 and SamCystin Interact. Biochemical and Biophysical Research Communications 383:16-21 (2009).

Bryda, E.C . and Riley, L.K. Multiplex Microsatellite Markers Panels for Genetic Monitoring of Common Rat Strains. Journal of the American Association for Laboratory Animal Science 47: 37-41 (2008).

Bouvrette, D.J., Price, S.J., Bryda, E.C. The K Homology Domains of the Mouse Polycystic Kidney Disease-related Protein, Bicaudal-C (Bicc1) Mediate RNA Binding In Vitro. Nephron Experimental Nephrology 108:27-34 (2008).

Bryda, E.C. , Pearson, M., Agca, Y., Bauer, B.A. Method for Detection and Identification of Multiple Chromosomal Integration Sites in Transgenic Animals Created with Lentivirus. Biotechniques 41:715-719 (2006).

Cogswell, C., Price, S. J., Hou, X., Guay-Woodford, L.M., Flaherty, L., Bryda, E.C. Positional cloning of the jcpk/bpk locus of the mouse. Mammalian Genome 14:242-249 (2003).

Bryda, E.C ., Kim, H.J., Legare, M.E., Frankel, W., Noben-Trauth, K. High resolution genetic and physical mapping of modifier-of-deafwaddler (mdfw) and waltzer (v). Genomics 73: 338-342 (2001).

Di Palma, F., Holme, R.H., Bryda, E.C. , Belyantseva, I.A., Pellegrino, R., Kachar, B., Steel, K.P., Noben-Trauth, K. Mutations in Cdh23, encoding a new type of cadherin, cause stereocilia disorganization in waltzer, the mouse model for Usher syndrome type 1D. Nature Genetics 27: 103-107 (2001).

Bolz, H., von Brederlow, B., Ramirez, A., Bryda, E.C. , Kutsche, K., Nothwang, H-G, Seeliger, M., Cabrera, M. d.C-S., Vila, M.C., Molina, O.P., Gal, A., Kubisch, C. Mutation of CDH23, encoding a new member of the cadherin gene family, causes Usher syndrome type 1D. Nature Genetics 27:108-112 (2001).

 

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